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vector red alkaline phosphatase (red ap) substrate kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vector red alkaline phosphatase (red ap) substrate kit
    Vector Red Alkaline Phosphatase (Red Ap) Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/red+alkaline+phosphatase+substrate+kit+i/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/custom%40sk-5100%4010%2E22203%2Fecm%2Ev033a11
    Average 96 stars, based on 1934 article reviews
    vector red alkaline phosphatase (red ap) substrate kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: The nuclear cap-binding complex safeguards stress-resistant protein synthesis and proliferation of stem cells
    Article Snippet: For NE differentiation, hESCs were cultured on Matrigel-coated plates in an hESC medium (DMEM/F12 supplemented with 15% knockout serum replacement, minimum essential medium nonessential amino acids and 0.1 mM 2-mercaptoethanol) added with 10 μM SB431542 (PeproTech) and 2 μM dorsomorphin (Tocris) via the dual SMAD inhibition method.

    Activity Assay:

    Article Title: Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans
    Article Snippet: .. ALP activity was detected with VECTOR Red Alkaline Phosphatase Substrate Kit I (Cat. SK-5100, Vector Laboratories) according to the manufacturer's instructions. ..

    Article Title: Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans
    Article Snippet: .. ALP activity was detected with VECTOR Red Alkaline Phosphatase Substrate Kit I (Cat. SK-5100, Vector Laboratories) according to the manufacturer's instructions. ..

    Article Title: Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans
    Article Snippet: .. On day 15, the cells were stained for ALP activity, using VECTOR Red Alkaline Phosphatase Substrate Kit I (Cat. SK-5100, Vector Laboratories), according to the manufacturer’s instructions, and the number of ALP-positive colonies were counted. .. To quantify gene expression in the mouse cells, the total RNA was extracted with RNeasy Plus Mini Kit (Cat. 74136, Qiagen) and reverse-transcribed with SuperScript III (Cat. 18080044, Thermo Fisher Scientific), using random primers (Cat. C1181, Promega).

    Article Title: Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans
    Article Snippet: .. On day 15, the cells were stained for ALP activity, using VECTOR Red Alkaline Phosphatase Substrate Kit I (Cat. SK-5100, Vector Laboratories), according to the manufacturer’s instructions, and the number of ALP-positive colonies were counted. .. To quantify gene expression in the mouse cells, the total RNA was extracted with RNeasy Plus Mini Kit (Cat. 74136, Qiagen) and reverse-transcribed with SuperScript III (Cat. 18080044, Thermo Fisher Scientific), using random primers (Cat. C1181, Promega).

    Staining:

    Article Title: Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans
    Article Snippet: .. On day 15, the cells were stained for ALP activity, using VECTOR Red Alkaline Phosphatase Substrate Kit I (Cat. SK-5100, Vector Laboratories), according to the manufacturer’s instructions, and the number of ALP-positive colonies were counted. .. To quantify gene expression in the mouse cells, the total RNA was extracted with RNeasy Plus Mini Kit (Cat. 74136, Qiagen) and reverse-transcribed with SuperScript III (Cat. 18080044, Thermo Fisher Scientific), using random primers (Cat. C1181, Promega).

    Article Title: Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans
    Article Snippet: .. On day 15, the cells were stained for ALP activity, using VECTOR Red Alkaline Phosphatase Substrate Kit I (Cat. SK-5100, Vector Laboratories), according to the manufacturer’s instructions, and the number of ALP-positive colonies were counted. .. To quantify gene expression in the mouse cells, the total RNA was extracted with RNeasy Plus Mini Kit (Cat. 74136, Qiagen) and reverse-transcribed with SuperScript III (Cat. 18080044, Thermo Fisher Scientific), using random primers (Cat. C1181, Promega).

    Article Title: RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site
    Article Snippet: Antigen retrieval for the slide specimens were performed using IHC-Tek epitope retrieval solution and steamer set (IHC World, LLC.). .. The slides were then immersed in 3% H 2 O 2 for 10 min to quench endogenous peroxidase followed by blocking with 10% goat serum for 1 h. Vectastain ABC-AP Kit (Vector Labs, Burlingame, CA) and Vector Red Alkaline Phosphatase Substrate Kit I (Vector Labs, Burlingame, CA) were used for tissue staining as per manufacture protocol. .. FXR1 primary antibody (Proteintech, Cat#13194-1-AP) was used at 1:200 dilution and cMYC primary antibody (Santacruz Biotechnology, Cat#sc-47694) at 1:100.

    Blocking Assay:

    Article Title: RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site
    Article Snippet: Antigen retrieval for the slide specimens were performed using IHC-Tek epitope retrieval solution and steamer set (IHC World, LLC.). .. The slides were then immersed in 3% H 2 O 2 for 10 min to quench endogenous peroxidase followed by blocking with 10% goat serum for 1 h. Vectastain ABC-AP Kit (Vector Labs, Burlingame, CA) and Vector Red Alkaline Phosphatase Substrate Kit I (Vector Labs, Burlingame, CA) were used for tissue staining as per manufacture protocol. .. FXR1 primary antibody (Proteintech, Cat#13194-1-AP) was used at 1:200 dilution and cMYC primary antibody (Santacruz Biotechnology, Cat#sc-47694) at 1:100.

    ALP Assay:

    Article Title: The nuclear cap-binding complex safeguards stress-resistant protein synthesis and proliferation of stem cells
    Article Snippet: .. Reprogrammed iPSC colonies were fixed with 4% paraformaldehyde for 15 min and subjected to an alkaline phosphatase assay by using the Vector Red Alkaline Phosphatase Substrate Kit I (Vector laboratories). .. HEK293T cells were transfected with the indicated plasmids using either the calcium phosphate method or Lipofectamine 2000 (Invitrogen).



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